Lipopolysaccharide inhibits triglyceride synthesis in dairy cow mammary epithelial cells by upregulating miR-27a-3p, which targets the PPARG gene

The fat content of milk determines the quality milk, and triglycerides are major components fat. Milk synthesis is regulated by many factors. Lipopolysaccharide (LPS) has been shown to inhibit in bovine mammary epithelial cells, but research on underlying mechanisms limited. MicroRNA (miRNA) involved physiological processes, there have few studies their regulation synthesis. In this study, we aimed investigate whether LPS upregulates miR-27a-3p, which targets PPARG, thereby inhibiting a dairy cow cell line (MAC-T). After stimulation MAC-T PPARG gene expression were inhibited. TargetScan software was used predict miRNA targeting miR-27a-3p selected as candidate. A dual luciferase reporter assay further confirmed connection between gene. To functions mimic inhibitors transfected into cells. mRNA protein levels PPAR-? negatively correlated with miR-27a-3p. Lipid droplet accumulation triglyceride also expression. Inhibition partially reversed LPS-induced decreases summary, our results reveal that can upregulating